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These parameters are osmolarity, the pH, the concentration of CO2 and O2, concentration of nutrients, water quality, energy sources and proteins. On the other hand, the culture media for the different stages must guarantee biophysical parameters of excellent quality. Oocytes with homogeneous cytoplasm and with at least three layers of cumulus oophorus cells are required. Not all collected oocytes will be used, since many of them present damages or abnormalities and therefore are not suitable for maturation and will not be competent. The quality of the oocytes depends, to a great extent, on the classification and selection once they are aspirated.
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Oocitos recién aspirados, listos para la clasificación Master’s in Sustainable Tropical Agriculture.
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